For two-photon polymerization, typically lasers at 780 nm with 100 .. 200 fs pulse width and 50 .. 500 mW average power are employed. Fiber lasers are an ideal choice because of their compactness,…
Non-linear microscopic techniques, such as 2-photon fluorescence microscopy or second harmonic generation (SHG) microscopy, have become key technologies in biological imaging, enabling…
Multi-photon microscopy in Neuroscience
Two-photon fluorescence microscopy is a key technology in biological imaging that enables three-dimensional, non-invasive studies of tissue on the micrometer…
Multi-photon microscopy in Neuroscience
Two-photon fluorescence microscopy is a key technology in biological imaging that enables three-dimensional, non-invasive studies of tissue on the micrometer…
Becker Wolfgang, Axel Bergmann, Alexander Jelzow, Antje Neubauer, Angelika Rück, Konrad Birkmeier, and Patrick Leisching. “Metabolic Imaging by Simultaneous 2-Photon FLIM of NAD(P)H and FAD.” In…