Fluorescence lifetime imaging (FLIM)
Two-photon fluorescence lifetime imaging (FLIM) can provide insights beyond standard fluorescence microscopy and non-linear microscopy by analyzing fluorescence…
For two-photon polymerization, typically lasers at 780 nm with 100 .. 200 fs pulse width and 50 .. 500 mW average power are employed. Fiber lasers are an ideal choice because of their compactness,…
Non-linear microscopic techniques, such as 2-photon fluorescence microscopy or second harmonic generation (SHG) microscopy, have become key technologies in biological imaging, enabling…
Multi-photon microscopy in Neuroscience
Two-photon fluorescence microscopy is a key technology in biological imaging that enables three-dimensional, non-invasive studies of tissue on the micrometer…
Multi-photon microscopy in Neuroscience
Two-photon fluorescence microscopy is a key technology in biological imaging that enables three-dimensional, non-invasive studies of tissue on the micrometer…