FemtoFiber ultra 920

Femtosecond fiber laser for 2-photon microscopy and neuroscience

• Brighter images with Clean-Pulse technology
• Fully turn-key with integrated AOM and GDD
• No noise-stress for animals thanks to fan-free passive cooling
• Compact laser design saving valuable table space
• Low cost of ownership using robust & reliable fiber-laser technology

In 2-photon microscopy, peak-power is brightness! If you care for the best image brightness, you need short pulses, high power, and most importantly a clean temporal pulse shape.
With TOPTICAs unique Clean-Pulse Technology, more than sufficient output power, and shortest pulses, the FemtoFiber ultra 920 features the highest relative peak power and enables unmatched brightness in 2-photon microscopy without unwanted heating of the sample.
Turn-key and intuitive operation, fully-integrated GDD and AOM make the system extremely user-friendly and allow you to focus on your research!
Without the need for regular maintenance or alignment TOPTICAs robust & reliable fiber laser technology significantly reduces the cost of ownership of your lasersystems. In addition, our quiet & compact laser design minimizes noise-stress to the animals while at the same time saving valuable table space.

The FemtoFiber ultra 920 is ideally suited for the two-photon excitation of common fluorophores like GFP, eGFP, Eosin, GCaMP, CFP, Calcein or Venus.

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Specification

Center wavelength	920 nm
Pulse duration	< 100 fs
Average output power	> 1.5 W
Repetition rate	80 MHz
Motorized dispersion precompensation (GDD)	-40000 .. +1000 fs²
Integrated power control (AOM, optional)	> 1 MHz AOM modulation bandwidth
Power in main pulse	> 98% (typ.)

Additional Information

Typical emission spectrum (linear).

Interferometric autocorrelation.

Retrieved pulse shape with typ. 99% of the laser power in main peak.
References

“The FemtoFiber ultra 920 was a pleasure to work with: it is incredibly compact and easy to use, and lowers the barrier to entry for two-photon microscopy. In particular, I appreciated the added features like integrated dispersion compensation and power control as they minimise the extra effort I need to put in when setting up a 2P microscope. Having years of experience in multiphoton microscopy, I feel that lasers like this one that we can just „plug and play“ will be game-changers for the field.“

„The inherent stability, the ease of use, and the clean temporal pulse shape of the FemtoFiber ultra 920 make TOPTICA‘s fiber lasers the ideal choise for our applications in neuroscience“

„For me the FemtoFiber ultra 920 with its Clean-Pulse Technology has the perfect optical parameters for highest two-photon image quality. In addition, the passive and cold laser head is an essential feature since it reduces the noise in laboratory and with that the stress on the living animals“

“I recommend the FemtoFiber ultra 920 and FemtoFiber ultra 1050 to other colleagues because both are reliable, easy to use, plug and play, with specifications that perfectly fit our purpose, no vibration on the optical table, no chiller noise, and last but not least because of the great support from TOPTICA.”

“I chose the FemtoFiber Ultra 920 due to its short pulse, compact design, and high power which is sufficient for two systems. So it's great, the performance is as promised, the laser is very stable and the beam profile is exceptionally clean. Highly recommended!“
Applications
- 2P-Microscopy
- Neuroscience
- SHG microscopy
Downloads
Literature
- Scientific Article: W. Zong, et al., Large-scale two-photon calcium imaging in freely moving mice, Cell https://doi.org/10.1016/j.cell.2022.02.017 (2022)
- Scientific Article: Ruihong Dai , et al., High energy (>40 nJ), sub-100 fs, 950 nm laser for two-photon microscopy, Optics Express, Vol. 29, Issue 24 (2021)
- Scientific Article: J. Han, et.al., Robust functional imaging of taste sensation with a Bessel beam, Biomedical Optics Express 12, 5855 (2021)
- Webinar: Setting Up a Simple and Cost-Efficient Two-Photon Microscope for Neuroscience
- Scientific Paper: Simplifying two-photon microscopy (2020)
- Article: TOPTICA Introduces FemtoFiber ultra 920 (2019)
- Application Note: Dr. Max Eisele, Bernhard Wolfring "Next generation two-photon microscopy using the FemtoFiber ultra 920 fiber laser", Physics Today (2019)
- Application Note: Dr. Max Eisele, Bernhard Wolfring "Next generation two-photon microscopy using the FemtoFiber ultra 920 fiber laser" (2019)

Beam shape	TEM₀₀, M² < 1.2
Beam divergence	< 1 mrad
Beam size (1/e²)	Ø 1.0 mm (typ.)
Linear polarization	100:1 (>20 dB), vertical
Output coupling	Free space
Dimensions laser head	77 x 165 x 300 mm³ (H x W x D)
Weight laser head	< 5 kg
Dimensions supply unit	131 x 484 x 600 mm³
Weight supply unit	< 20 kg
Power supply	24V DC (AC power supply included)
Power consumption	< 150 W
PC Interface	Ethernet, USB
Environment temperature	19 - 25 °C (operating), 0 - 40 °C (storage and transport)
Environment humidity	Non-condensing