iChrome MLE

Powerful 4-line laser combiner

Make no compromises on laser power to push the limits of super resolution and spinning disk microscopy

iChrome MLE

Powerful 4-line laser combiner

The iChrome MLE combines up to 3 different diode lasers and one DPSS laser fully integrated in one compact housing. The individual lasers are efficiently coupled and delivered via an all-in-one PM/SM fiber output. The microprocessor controlled system enables flexible OEM integration. High speed analog and digital modulation allow fast switching of laser wavelength and intensity. The unified user interface enables easy and efficient integration into any application.
TOPTICA’s ingenious COOL^AC technology automatically aligns the system with a single push of a button. This feature ensures a constant optical output level even under strongly varying ambient conditions and completely eliminates the need for manual realignment - making the iChrome MLE the most advanced multi-line laser system on the market.

Your Benefits

Reliable and long term stable

With COOL^AC automatic alignment you will never again waste valuable time for manual laser re-alignment. The iChrome MLE single-mode fiber coupled laser-engine is made to support your research needs.

Fast modulation capabilities

iChrome MLE laser systems can modulate up to 20 MHz for all lines (including the DPSS laser line) to serve even the most demanding applications.

Applications

Confocal Microscopy

In confocal microscopy (LSCM), tightly focused laser beams scan biological specimens point by point, generating high-contrast optical sections with exceptional spatial resolution. Our stable, low-noise laser sources with direct modulation of all wavelengths ensure optimal excitation efficiency, no optical cross-talking and minimal photobleaching — key for long-term imaging in live-cell studies.

Lightsheet microscopy (SPIM)

Lightsheet microscopy (SPIM) takes optical sectioning to the next level by illuminating samples with a thin sheet of light, reducing phototoxicity while achieving fast 3D imaging. With our multi-line laser platforms, researchers can simultaneously excite multiple fluorophores to capture rich volumetric data of developing embryos, organoids, and living tissues.

Super resolution microscopy (STED, SIM, PALM, STORM)

Pushing beyond the diffraction limit, super-resolution techniques such as STED, SIM, PALM, and STORM demand lasers with exceptional stability, pulse control, high power levels and spectral purity.

Optical scatterometry (OCD)

As semiconductor devices approach atomic-scale dimensions, controlling and verifying every nanometer of pattern geometry becomes a decisive factor for yield and performance. Traditional inspection tools like electron microscopy deliver high resolution, but they are too slow, too localized, and often destructive for in-line process control. Optical Scatterometry, also known as Optical Critical Dimension (OCD) metrology, provides the essential bridge between high-resolution analysis and high-throughput manufacturing. By analyzing how laser light is scattered, diffracted, or reflected from micro- and nanostructures, OCD enables non-contact, rapid, and precise measurement of line width, height, sidewall angle, and layer thickness — with sub-nanometer accuracy.

Interferometry

In semiconductor manufacturing, perfection is measured in nanometers - or often even less. To measure such minute variations with speed and accuracy, the industry relies on laser-based interferometric metrology - a non-contact, non-destructive optical technique capable of resolving surface and layer thickness with sub-nanometer precision.

Options

iChrome CLE

Compact and efficient four-color laser engine

iChrome FLE

Flexible Multi-color laser combiner

iChrome MLE

Powerful four-color laser engine

Specifications

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Downloads

Technical Drawings

Technical drawings iChrome MLE

Literature

Articles

Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia

Young-Mee Kim, et al., Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia, Nature Cancer volume 6, pages1350 1369 (2025)
Chip-based label-free incoherent super-resolution optical microscopy

Nikhil Jayakumar, et al., Chip-based label-free incoherent super-resolution optical microscopy, Nature, Light: Science & Applications 14, Article number 259 (2025)
iChrome MLE: More than just Four Lasers in a Box

iChrome MLE: More than just four lasers in a box, Wiley (2014)
Klaviatur der Farben

Dr. Marion Lang, Dr. Thomas Renner, Klaviatur der Farben, Laser (2011)
Lasers for Confocal Microscopy

Dr. Marion Lang, Lasers for Confocal Microscopy, Imaging & Microcopy (2011)

Papers

Bayesian Inference of Binding Kinetics from Fluorescence Time Series

J. Shepard Bryan IV et al., Bayesian Inference of Binding Kinetics from Fluorescence Time Series (2025)

Application Notes

A multicolor TIRF microscope

Dr. Marion Lang et al., A multicolor TIRF microscope (2012)

iChrome MLE

iChrome MLE

Your Benefits

Applications

Options

Specifications

Related Products

Downloads

Technical Drawings

Literature

Articles

Papers

Application Notes