iChrome FLE
Fluorescence microscopy deserves unlimited flexibility, fast switching and all laser wavelengths from a single box
iChrome FLE
Laser combiners
TOPTICA's iChrome FLE is a flexible laser engine that combines up to 7 laser lines in up to two polarization-maintaining single-mode fibers. Power levels reach up to 100 mW per laser line. The flexible setup can be customized to meet the special needs of each customer. The standard wavelength range of the FLE is 405 to 780 nm. We will select the appropriate fiber according to your desired wavelengths. If the entire wavelength range is required, we can fully integrate a PC-Fiber (Photonic Crystal Fiber), to cover the full wavelength range of 405 to 780 nm. Like all iChrome systems, the FLE guarantees a plug & play installation via TOPTICA's proprietary COOLAC automatic alignment technology.
The iChrome FLE is designed and optimized for many different microscopy techniques. The modulation behavior has been thoroughly tested and optimized at TOPTICA, to guarantee the best performance for scanning applications. Its power level and wavelength range also make it a perfect choice for many non-scanning applications, such as light-sheet microscopy (also known as SPIM), TIRF, spinning disk, structured illumination, super-resolution microscopy and many others.
COOLAC gives the laser combiner plug-and-play simplicity. Installation is done with the simple click of a button in the user software.
Green wavelength laser light (532*, 561 and 594 nm) is generated by TOPTICA´s proprietary FDDL (Frequency Doubled Diode Laser) technology, an all-diode approach for these very important wavelengths. Each laser line, including the FDDL’s, is directly modulated up to a frequency of 1 MHz and TOPTICA's "Complete OFF" mechanism guarantees zero photons in the off state. “Complete OFF” also means, that there is absolutely no crosstalk between the individual wavelengths.
The iChrome FLE is designed and optimized for many different microscopy techniques. The modulation behavior has been thoroughly tested and optimized at TOPTICA, to guarantee the best performance for scanning applications. Its power level and wavelength range also make it a perfect choice for many non-scanning applications, such as light-sheet microscopy (also known as SPIM), TIRF, spinning disk, structured illumination, super-resolution microscopy and many others.
COOLAC gives the laser combiner plug-and-play simplicity. Installation is done with the simple click of a button in the user software.
Green wavelength laser light (532*, 561 and 594 nm) is generated by TOPTICA´s proprietary FDDL (Frequency Doubled Diode Laser) technology, an all-diode approach for these very important wavelengths. Each laser line, including the FDDL’s, is directly modulated up to a frequency of 1 MHz and TOPTICA's "Complete OFF" mechanism guarantees zero photons in the off state. “Complete OFF” also means, that there is absolutely no crosstalk between the individual wavelengths.
Your Benefits
Tailored for you
With up to 7 laser lines you achieve almost unlimited flexibility. Tailored to your application needs.
Reliable and long term stable
With COOLAC automatic alignment you will never again waste valuable time for manual laser re-alignment. The iChrome FLE is always perfectly aligned.
Increased contrast and reduced cross-talk
With their superior modulation capabilities the iChrome laser systems are ideal for the most demanding modulation schemes used in microscopy.
Options
iChrome CLE
Compact and efficient four-color laser engine
iChrome FLE
Flexible Multi-color laser combiner
iChrome MLE
Powerful four-color laser engine
Separate fiber for dedicated wavelengths
An option available with the iChrome FLE is a separate fiber for up to two wavelengths. TOPTICA’s optical design allows two lasers to be carried in an additional fiber. These could be special wavelengths designed to run in a separate beam path (e.g. 405 or 780 nm, which are corrected separately within the microscope). Another option is to double an existing wavelength for simultaneous high-power operation (e.g. 405 or 488 nm for a separate FRAP input of a microscope).
2 fibers with fiber-splitter and fiber switch
Systems equipped with two laser fibers benefit from TOPTICA’s fiber switching technology, which combines fiber switching and fiber splitting in one option. Power hungry applications can benefit from the full power levels at the corresponding fiber, while applications such as a two light-sheet setup can benefit from an exact power balance between both fibers.
Related Products
Downloads
Literature
Articles
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Demand-tailored laser systems: Integrated multi-color laser solutions for quantum optics and biophotonics
Konstantin Birngruber, Stephan Falke Demand-tailored laser systems: Integrated multi-color laser solutions for quantum optics and biophotonics, Physik Journal Physics’ Best 2019 -
Bedarfsoptimierte Lasersysteme - Integrierte Multi-Farben-Laserlösungen für Quantenoptik und Biophotonik
Konstantin Birngruber, Stephan Falke Bedarfsoptimierte Lasersysteme - Integrierte MultiFarben-Laserlösungen für Quantenoptik und Biophotonik, Physik Journal Best of 2018 -
Four‐Color Laser Engine for Efficient Confocal Microscopy
Tim Paasch-Colberg, Konstantin Birngruber, Four‐Color Laser Engine for Efficient Confocal Microscopy, Optik & Photonik (2018) -
Klaviatur der Farben
Dr. Marion Lang, Dr. Thomas Renner, Klaviatur der Farben, Laser (2011) -
Lasers for Confocal Microscopy
Dr. Marion Lang, Lasers for Confocal Microscopy, Imaging & Microcopy (2011)