Simplifying multi-photon microscopy with stress-less femtosecond lasers
FemtoFiber ultra 920
Femtosecond fiber laser
In two-photon microscopy, peak-power is brightness! If you care for the best image brightness, you need short pulses, high power, and most importantly a clean temporal pulse shape. With TOPTICAs unique Clean Pulse Technology, the FemtoFiber ultra 920 enables unmatched brightness in two-photon microscopy without unwanted heating of the sample.
Additionally, software-controlled dispersion precompensation (GDD) and an integrated AOM for power modulation, make the system extremely user-friendly.
TOPTICAs robust & reliable fiber laser technology significantly reduces the cost of ownership of your laser systems. In addition, our quiet & compact laser design minimizes noise-stress to the animals while at the same time saving valuable table space.
The FemtoFiber ultra 920 is ideally suited for the two-photon excitation of common fluorophores like GFP, eGFP, Eosin, GCaMP, CFP, Calcein or Venus.
Image Gallery
Your Benefits
Compact & Low cost of ownership
Saving table space with all-integrated robust fiber laser system.
Clean Pulse Technology
TOPTICA’s unique Clean Pulse Technology provides clean, pedestal-free pulses even after the fiber for best image brightness.
Images by Shau Poh Chong and Peter Török
User-friendly GDD
Short pulses and software-controlled dispersion precompensation (GDD) for user-friendly handling TOPTICA’s FemtoFiber ultra provides motorized group delay dispersion (GDD) pre-compensation that can be controlled in software. Due to chromatic dispersion, a femtosecond pulse stretches in time when transmitted through optical material in the microscope. GDD pre-compensation adds the opposite dispersion to circumvent this effect by cancelling it out. TOPTICA’s software-controllable GDD pre-compensation ensures short pulses at the sample plane with user-friendly, repeatable optimization of fluorescence signal strength via the graphical user-interface (GUI).
Fast power control
An integrated acousto-optic modulator (AOM) enables fast power-modulation and flyback blanking in synchronization with the beam scanner. This minimizes sample damage and photo-bleaching. Also, a fast electronic trigger output is available as reference for TCSPC in FLIM and gated detection.
For all important fluorophores
The FemtoFiber ultra 920 is ideally suited for the two-photon excitation of common fluorophores like GFP, GCaMP, RFP, mCherry, tdTomato, BFP, Alexa, NADH, FAD, auto-fluorescence or SHG-imaging.
More than 20 years of experience
With 20 years of experience developing OEM-class fiber lasers, TOPTICA is a leader in the field. We have tailored our FemtoFiber ultra series to provide customers with an industrial-grade light engine for high-end applications. Unlike bulky, alignment-sensitive Ti:Sapphire or OPO systems, TOPTICA's femtosecond fiber lasers are reliable and compact. They provide femtosecond pulses with high average power and excellent temporal and spatial beam quality.
Applications
Neuroscience
High peak power and clean pulse shape are needed for deep-tissue imaging.
Other microscopy types (TIRF, FLIM, ...)
Modern biophotonics goes far beyond conventional fluorescence or confocal microscopy. Researchers today explore living systems through light-matter interactions that reveal chemical composition, molecular dynamics, mechanics, and function. All without labels or stains.
Two-Photon microscopy
Two-photon excitation microscopy opens a window deep into living tissue.
Lithography & microfabrication
From semiconductor patterning and 3D microprinting to holographic optics and transparent displays, laser light is the most precise tool.
Options
AOM
Integrated AOM for fast modulation of the laser light, integrated to the system.
Pulse picker
An integrated pulse picker reduces the effective repetition rate from 80 MHz to 8 MHz (1:10).
Custom repetition rate
The custom repetition rate provides a modified oscillator with a pre-defined repetition rate between 40 and 80 MHz upon ordering.
Dual-color
With the FemtoFiber ultra dual-color option two FemtoFiber ultra are optically synchronized and allow simultaneous imaging with two different laser colors.
No laser wavelength tuning is required.
FemtoFiber ultra 460
The system has an additional SHG unit that can generate femtosecond pulses with an average power of >0.1 W at 460 nm.
Fiber delivery
Replace the free-space path between the femtosecond laser and the two-photon microscope. The FemtoFiber ultra FD or Mini2P variant delivers its pulses via a polarization-maintaining hollow-core fiber.
The FemtoFiber ultra is a very powerful laser system that exceeded our requirements. We never maxed out the capabilities of the laser system.
Prof. Dr. Jörg Enderlein
Georg-August-Universität Göttingen, III. Physikalisches Institut, Germany
As an independent neuroscience microscope integrator INSS has installed numerous multi-photon systems around the globe. We integrated many TOPTICA FemtoFiber ultra lasers in different configurations. FemtoFiber ultra 920 is a rock-solid solution for two-photon excitation of GCaMP and other fluorescent protein.
Bruno Pichler, Ph.D.
Founder INSS
I chose the FemtoFiber ultra 920 due to its short pulse, compact design, and high power which is sufficient for two systems. So it's great, the performance is as promised, the laser is very stable and the beam profile is exceptionally clean. Highly recommended!
Prof. Yoav Adam
The Hebrew University, Israel
The inherent stability, the ease of use, and the clean temporal pulse shape of the FemtoFiber ultra 920 make TOPTICA‘s fiber lasers the ideal choice for our applications in neuroscience.
Zhou Zhenqiao, Ph.D.
Suzhou Institute of Biomedical Engineeering and Technology (SIBET), China
I recommend the FemtoFiber ultra 920 and FemtoFiber ultra 1050 to other colleagues because both are reliable, easy to use, plug and play, with specifications that perfectly fit our purpose, no vibration on the optical table, no chiller noise, and last but not least because of the great support from TOPTICA.
