In two-photon microscopy, peak-power is brightness! If you care for the best image brightness, you need short pulses, high power, and most importantly a clean temporal pulse shape. With TOPTICAs unique Clean Pulse Technology, the FemtoFiber ultra 920 enables unmatched brightness in two-photon microscopy without unwanted heating of the sample.
Additionally, software-controlled dispersion precompensation (GDD) and an integrated AOM for power modulation, make the system extremely user-friendly.
TOPTICAs robust & reliable fiber laser technology significantly reduces the cost of ownership of your laser systems. In addition, our quiet & compact laser design minimizes noise-stress to the animals while at the same time saving valuable table space.
The FemtoFiber ultra 920 is ideally suited for the two-photon excitation of common fluorophores like GFP, eGFP, Eosin, GCaMP, CFP, Calcein or Venus.
Your Benefits
Brighter images with Clean Pulse technology
TOPTICA’s unique Clean Pulse Technology provides clean, pedestal-free and short pulses for best image brightness.
The combination of a high temporal pulse quality (a “clean pulse”) and a short pulse duration at the sample plane gives the best result in terms of overall 2-photon excitation.
Hence, to achieve the best image quality, TOPTICA’s FemtoFiber ultra 920 incorporates Clean Pulse Technology - a laser design that significantly reduces pedestals and side-wings on the temporal pulse profile compared to other laser amplifier designs. Thus, the full laser power of the FemtoFiber ultra 920 is contained in the main laser pulse to maximize two-photon excitation. With Clean Pulse Technology, the FemtoFiber ultra 920 enables unmatched fluorescence image brightness in non-linear microscopy (see Literature).
Compact laser design
Compact laser design saving valuable table space
he laser head includes all free-space optics of the system, holding them in a compact housing. No active or passive cooling is required and the laser head can be mounted horizontally on the optical table or vertically against a wall. Due to the passive cooling, no noise-stress for animals is introduced.
The laser control unit is connected by an umbilical cable and can be placed in a Rack or below the optical table apart from the user's set-up.
User-friendly GDD
Short pulses and software-controlled dispersion precompensation (GDD) for user-friendly handling
TOPTICA’s FemtoFiber ultra provides motorized group delay dispersion (GDD) pre-compensation that can be controlled in software. Due to chromatic dispersion, a femtosecond pulse stretches in time when transmitted through optical material in the microscope. GDD pre-compensation adds the opposite dispersion to circumvent this effect by cancelling it out. TOPTICA’s software-controllable GDD pre-compensation ensures short pulses at the sample plane with user-friendly, repeatable optimization of fluorescence signal strength via the graphical user-interface (GUI).
Fast power control
An integrated acousto-optic modulator (AOM) enables fast power-modulation and flyback blanking in synchronization with the beam scanner. This minimizes sample damage and photo-bleaching. Also, a fast electronic trigger output is available as reference for TCSPC in FLIM and gated detection.
For all important fluorophores
The FemtoFiber ultra 920 is ideally suited for the two-photon excitation of common fluorophores like GFP, GCaMP, RFP, mCherry, tdTomato, BFP, Alexa, NADH, FAD, auto-fluorescence or SHG-imaging.
Low cost of ownership
Robust & reliable fiber laser technology significantly reduces the cost of ownership. The laser systems require no chiller and have low power consumption. In addition, the quiet & compact laser design minimizes noise-stress to the animals while at the same time saving valuable table space.
More than 20 years of experience
TOPTICA is drawing upon 20 years of experience in developing OEM-class fiber lasers. We have tailored our FemtoFiber ultra series to provide our customers with an industrial-grade light engine for high-end applications. The reliable and compact laser design provides femtosecond pulses with high average power, excellent temporal and spatial beam quality.
Applications
Neuroscience
Our femtosecond pulsed lasers deliver the high peak power and wavelength flexibility needed for deep-tissue imaging in neuroscience, where precise optical stimulation and recording reveal the secrets of brain function and connectivity.
Other microscopy types (TIRF, FLIM, ...)
Modern biophotonics goes far beyond conventional fluorescence or confocal microscopy. Researchers today explore living systems through light-matter interactions that reveal chemical composition, molecular dynamics, mechanics, and function. All without labels or stains.
Two-Photon microscopy
Two-photon excitation microscopy opens a window deep into living tissue, allowing researchers to probe neuronal circuits with minimal damage.
Lithography & microfabrication
From semiconductor patterning and 3D microprinting to holographic optics and transparent displays, laser light is the most precise tool.
Options
AOM
Integrated AOM for fast modulation of the laser light, integrated to the system.
Pulse picker
Integration of pulse-picker for adjustable repetition rate. The pulse-picker picks single pulses from the oscillator from every second pulse down to every tenth pulse only.
Custom repetition rate
Fixed custom repitition rate provides a modified oscillator with an upon ordering pre-defined repetition rate.
Dual-color
With the FemtoFiber ultra dual-color option two FemtoFiber ultra are optically synchronized and allow simultaneous imaging with two different laser colors. No laser wavelength tuning is required. A shared oscillator design guarantees all-optical synchronization and hence a fixed, defined delay between the two laser colors with minimum jitter.
The Femto Fiber ultra can also be optically synchronized to the FemtoFiber dichro, FemtoFiber smart or TeraFlash pro.
FemtoFiber ultra 460
System with additional SHG united factory-premounted to the system, allowing to generate 460 nm femtosecond pulses, but also being switchable to 920 nm
The FemtoFiber ultra is a very powerful laser system that exceeded our requirements. We never maxed out the capabilities of the laser system.
Prof. Dr. Jörg Enderlein
Georg-August-Universität Göttingen, III. Physikalisches Institut, Germany
As an independent neuroscience microscope integrator INSS has installed numerous multi-photon systems around the globe. We integrated many TOPTICA FemtoFiber ultra lasers in different configurations. FemtoFiber ultra 920 is a rock-solid solution for two-photon excitation of GCaMP and other fluorescent protein.
