Simplifying microscopy with a stress-less femtosecond laser for Mini2P
FemtoFiber ultra Mini2P
Femtosecond fiber laser for Mini2P
In the FemtoFiber ultra Mini2P, TOPTICA combines a polarization-maintaining hollow-core fiber suitable for Mini2P (miniaturized 2-photon microscope) with TOPTICA’s proprietary COOL technology: COOLAC offers hands-off, automated fiber coupling that eliminates manual alignment at installation, optimizes fiber coupling efficiency at the touch of a button, and provides a safeguard monitor to make misalignment an impossibility. With COOLAC, exchange of the Mini2P-fiber is easy and fast, and can be peformed by the user.
Additionally, an integrated AOM for fast power modulation, software-controlled dispersion precompensation (GDD) and TOPTICA’s unique Clean Pulse Technology complete the laser system.
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Your Benefits
No mirror alignment
Just automated fiber coupling. TOPTICA's COOL technology saves users time and effort with its automated fiber coupling (COOLAC).
Simple fiber exchange
With COOLAC, exchange of the Mini2P-fiber is easy and fast, and can be performed by the user. Easy and fast swap to a standard FD-fiber is also possible.
Clean Pulse Technology
TOPTICA’s unique Clean Pulse Technology provides clean, pedestal-free pulses even after the fiber for best image brightness.
Images by Shau Poh Chong and Peter Török
Fast power control
An integrated acousto-optic modulator (AOM) enables fast power-modulation and flyback blanking in synchronization with the beam scanner. This minimizes sample damage and photo-bleaching. Also, a fast electronic trigger output is available as reference for TCSPC in FLIM and gated detection.
Main fluorophores addressed
The FemtoFiber ultra Mini2P is ideally suited for the two-photon excitation of common fluorophores like GFP, GCaMP, or JEDI-2P used in invivo studies.
Low cost of ownership
Robust & reliable fiber laser technology significantly reduces the cost of ownership. The laser systems require no chiller and have low power consumption. In addition, the quiet & compact laser design minimizes noise-stress to the animals while at the same time saving valuable table space.
More than 20 years of experience
TOPTICA is drawing upon 20 years of experience in developing OEM-class fiber lasers. We have tailored our FemtoFiber ultra series to provide our customers with an industrial-grade light engine for high-end applications. The reliable and compact laser design provides femtosecond pulses with high average power, excellent temporal and spatial beam quality.
Applications
Neuroscience
High peak power and clean pulse shape are needed for deep-tissue imaging.
Two-Photon microscopy
Two-photon excitation microscopy opens a window deep into living tissue.
Other microscopy types (TIRF, FLIM, ...)
Modern biophotonics goes far beyond conventional fluorescence or confocal microscopy. Researchers today explore living systems through light-matter interactions that reveal chemical composition, molecular dynamics, mechanics, and function. All without labels or stains.
Options
Pulse picker
An integrated pulse picker reduces the effective repetition rate from 80 MHz to 8 MHz (1:10).
Custom repetition rate
The custom repetition rate provides a modified oscillator with a pre-defined repetition rate between 40 and 80 MHz upon ordering.
For me the FemtoFiber ultra 920 with it's Clean-Pulse Technology has the perfect optical parameters for highest two-photon image quality. In addition, the passive and cold laser head is an essential feature since it reduces the noise in laboratory and with that the stress for living animals.
Dr. Weijiang -Zong
Moser Group, Kavli Institute for Systems Neuroscience, Norway
Konrad Birkmeier, et al., Femtosecond fiber delivery at 920 nm for two-photon microscopy, Proc. SPIE 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, 1284703 (12 March 2024)
