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Application Focus: Lasers for Microscopy

ǀ  TOPTICA Tuesday

Lasers play an invaluable role in instrumentation for biophotonics. Especially microscopy applications have a high demand for modern photonic light sources. Many TOPTICA lasers were developed with a strong focus on microscopy techniques. They fulfill the challenging requirements of these applications regarding their light properties, reliability, ease of use and longevity.

TOPTICA's portfolio includes UV, VIS and NIR laser sources, flexible multi-laser engines and pulsed fiber lasers. Here is an overview of microscopy techniques and the ideal laser sources to realize them:

Confocal Microscopy

iBeam smart, iChrome CLE

SPIM / SIM

iChrome CLE, iChrome MLE, iBeam smart

TIRF

iChrome MLE

FRAP

iChrome MLE with dual-fiber option

Spinning Disk

iChrome MLE, iBeam smart

Superresolution

iChrome MLE, iBeam smart

Live-cell imaging

iChrome CLE

Raman microscopy

XTRA II, iBeam smart WS, TopMode

SHG / THG microscopy

FemtoFiber pro, FemtoFiber ultra

CARS / SRS

Combination of FemtoFiber ultra and FemtoFiber pro

FLIM

FemtoFiber pro TVIS

Pulsed STED

FemtoFiber ultra