FemtoFiber Technology for Life Sciences and Microscopy
- Two-photon fluorescence of granule cells stained with dTomato (Courtesy of Alfred Leitenstorfer).
- Two-photon fluorescence
- Confocal microscopy
- FLIM, CARS, THG
- SHG Microscopy
- Wide tunability 488 nm – 2200 nm
- Femtosecond and picosecond pulses
- Synchronized two color beams
Modern biomedical research often involves advanced laser microscopy. Especially confocal laser scanning microscopy (LSM) attracts broad attention. The capability of optical sectioning and reconstruction of the sample’s 3D structure boosted the research on the single cell level. The on-going development of a huge variety of fluorescent markers enabled this break trough. Among the newest generation of markers are GFP and its derivates CFP, YFP, etc. In contrast to artificial dyes the GFP family is expressed inside and by the living cells themselves and attached to proteins according to the geneticist’s design. In parallel to this exciting development also the light sources for LSM kept pace. Tunable lasers account for the variety of fluorescent labels. Pulsed lasers enable fluorescent lifetime imaging microscopy (FLIM). And ultrafast lasers open the door for non-invasive methods relying on multi-photon excitation such as SHG, THG or CARS microscopy.
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While the techniques become more advanced, the employed lasers should become easier to handle. That's why TOPTICA's fiber lasers are the product of choice. Reliable and comfortable in handling, they allow the user to focus on the scientific task. Especially the iChrome is a fully automated and fiber coupled laser designed for confocal microscopy. It covers the entire visible range from 488 nm to 640 nm. With 40 MHz repetition rate it is perfectly suited for FLIM: In contrast to pulsed diode lasers it shows no after-ringing which can disturb the measurement. The FemtoFiber pro series allows for multi-photon and non-linear microscopy techniques: Femtosecond pulses covering wavelengths from 780 nm up to 1400 nm give access to new red markers like dTomato or two-photon excitation fluorescence (TPEF). Techniques like SHG and THG have been successfully demonstrated by our customers. Two or three synchronized and tunable outputs deliver picosecond pulses that are perfectly suited for CARS or SRS.
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Related Literature
Related Literature
- Brochures: Ultrafast Fiber Lasers
- Publications: Femtosecond Lasers for Life Sciences
- Publications: Highly versatile confocal microscopy system based on a tunable femtosecond Er : fiber source
- Publications: Compact coherent anti-Stokes Raman scattering microscope based on a picoseconds two-color Er:fiber laser system
- Publications: Fiber-format CARS spectroscopy by spectral compression of femtosecond pulses from a single laser oscillator
- Publications: Fiber-format stimulated-Raman-scattering microscopy from a single laser oscillator
