Microscopy with Diode Lasers

Advanced microscopy makes use of multi color laser illumination.
Advanced microscopy makes use of multi color laser illumination.

Nowadays diode lasers, fiber lasers and solid state lasers play the major role in modern confocal imaging instruments and have taken the part of formerly used gas lasers almost completely. For being a successful contributor in this fast moving field of optical imaging techniques, one has to cope quickly and reliably with the many special needs of the demanding customer base: perfect beam quality, high lifetime, high speed modulation of the sources and accessing virtually any wavelength in the UV / VIS / NIR region.

TOPTICAs diode lasers, offer high beam quality, high power stability and a superb lifetime at a very economic price. Furthermore, our diode lasers show direct modulation capabilities, with superior performance to any AOM of AOTF. Thus investment costs and optical system complexity are reduced. 

Our diode lasers provide a diffraction-limited beam with an excellent wavefront being the base for excellent resolution of confocal images. Always having the user in mind, TOPTICA has introduced unique features that make our iBeam smart lasers the preferred choice for the Biophotonic field:

  • FINE: The first purely electronic Feedback Induced Noise Eraser (FINE)
  • SKILL: TOPTICAs Speckle KILLer effectively reduces laser speckle, without adding any moving parts (no focal blurring).


As laser diodes are available at various distinct wavelengths (from 375 nm to 830 nm), researchers can select their preferred wavelength and do not need to compromise anymore and allow exciting even exotic dyes (e.g. NIR dyes).

Of course, TOPTICA also offers high quality, convenient single-mode and polarization maintaining fiber delivery, optimized for each wavelength.

Related Products

Related Products

Related Literature

Related Literature

Brochure: iChrome MLE
Brochure: iChrome MLE
TOPTICA_12_iChrome_MLE.pdf
Brochure: iBeam smart
Brochure: iBeam smart
TOPTICA-iBeamSmart_A4_2011.pdf
Brochure: iBeam smart PT
Brochure: iBeam smart PT
TOPTICA_iBeamPT_A4.pdf
Brochure: Ultrafast Fiber Lasers
Brochure: Ultrafast Fiber Lasers
toptica_BR_Ultrafast_Fiber_Lasers_16.pdf
Brochure: Lasers for Biophotonics
Brochure: Lasers for Biophotonics
toptica_BR_biophotonics_lasers_2008_07.pdf
Application Note: A multicolor TIRF microscope
Application Note: A multicolor TIRF microscope
toptica_AP_1013_A_multicolor_TIRF_microscope.pdf
Article: Lasers for Confocal Microscopy
Article: Lasers for Confocal Microscopy
toptica_lang_imaging_microscopy_2011.pdf
Article: Klaviatur der Farben
Article: Klaviatur der Farben
1105_LASER_Klaviatur_der_Farben.pdf

Related Applications

Related Applications

  • Fluorescence detection and imaging
  • Confocal microscopy
  • Raman microscopy
  • FLIM, FRET, FRAP etc.
  • CARS microscopy
  • Multi photon microscopy
  • Wide field imaging techniques (e.g. TIRF)
  • Super resolution imaging including STED, PALM, STORM
  • Raman spectroscopy and microscopy